Biofunctionalization of Polyoxometalates with DNA Primers, Their Use in the Polymerase Chain Reaction (PCR) and Electrochemical Detection of PCR Products.

Ahmed M Debela, Mayreli Ortiz, Valerio Beni, Serge Thorimbert, Denis Lesage, Richard B Cole, Ciara K O'Sullivan, Bernold Hasenknopf

Chemistry (Weinheim an der Bergstrasse, Germany) 2015 Dec 1

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The bioconjugation of polyoxometalates (POMs), which are inorganic metal oxido clusters, to DNA strands to obtain functional labeled DNA primers and their potential use in electrochemical detection have been investigated. Activated monooxoacylated polyoxotungstates [SiW11 O39 {Sn(CH2 )2 CO}](8-) and [P2 W17 O61 {Sn(CH2 )2 CO}](6-) have been used to link to a 5'-NH2 terminated 21-mer DNA forward primer through amide coupling. The functionalized primer was characterized by using a battery of techniques, including electrophoresis, mass spectrometry, as well as IR and Raman spectroscopy. The functionality of the POM-labeled primers was demonstrated through hybridization with a surface-immobilized probe. Finally, the labeled primers were successfully used in the polymerase chain reaction (PCR) and the PCR products were characterized by using electrophoresis. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Ahmed M Debela, Mayreli Ortiz, Valerio Beni, Serge Thorimbert, Denis Lesage, Richard B Cole, Ciara K O'Sullivan, Bernold Hasenknopf. Biofunctionalization of Polyoxometalates with DNA Primers, Their Use in the Polymerase Chain Reaction (PCR) and Electrochemical Detection of PCR Products. Chemistry (Weinheim an der Bergstrasse, Germany). 2015 Dec 1;21(49):17721-7