Morpholinos: Antisense and Sensibility.

Martin Blum, Edward M De Robertis, John B Wallingford, Christof Niehrs

Developmental cell 2015 Oct 26

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For over 15 years, antisense morpholino oligonucleotides (MOs) have allowed developmental biologists to make key discoveries regarding developmental mechanisms in numerous model organisms. Recently, serious concerns have been raised as to the specificity of MO effects, and it has been recommended to discontinue their usage, despite the long experience of the scientific community with the MO tool in thousands of studies. Reviewing the many advantages afforded by MOs, we conclude that adequately controlled MOs should continue to be accepted as generic loss-of-function approach, as otherwise progress in developmental biology will greatly suffer. Copyright © 2015 Elsevier Inc. All rights reserved.

Citation

Martin Blum, Edward M De Robertis, John B Wallingford, Christof Niehrs. Morpholinos: Antisense and Sensibility. Developmental cell. 2015 Oct 26;35(2):145-9