Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions.

Natsumi Maruta, Yuri Trusov, Jose R Botella

Methods in molecular biology (Clifton, N.J.) 2016

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Protein-protein interaction studies provide useful insights into biological processes taking place within the living cell. A number of techniques are available to unravel large structural protein complexes, functional protein modules, and temporary protein associations occurring during signal transduction. The choice of method depends on the nature of the proteins and the interaction being studied. Here we present an optimized and simplified yeast three-hybrid method for analysis of protein interactions involving three components.

Citation

Natsumi Maruta, Yuri Trusov, Jose R Botella. Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions. Methods in molecular biology (Clifton, N.J.). 2016;1363:145-54