Correlation Engine 2.0
Clear Search sequence regions

  • cellular process (1)
  • cytosol (1)
  • Drp1 (14)
  • Dynamins (2)
  • gtpase (3)
  • impairs (2)
  • lipid (1)
  • Mff (10)
  • mice (1)
  • mutant proteins (2)
  • weak (1)
  • Sizes of these terms reflect their relevance to your search.

    Mitochondrial fission is a crucial cellular process mediated by the mechanoenzymatic GTPase, dynamin-related protein 1 (Drp1). During mitochondrial division, Drp1 is recruited from the cytosol to the outer mitochondrial membrane by one, or several, integral membrane proteins. One such Drp1 partner protein, mitochondrial fission factor (Mff), is essential for mitochondrial division, but its mechanism of action remains unexplored. Previous studies have been limited by a weak interaction between Drp1 and Mff in vitro. Through refined in vitro reconstitution approaches and multiple independent assays, we show that removal of the regulatory variable domain (VD) in Drp1 enhances formation of a functional Drp1-Mff copolymer. This protein assembly exhibits greatly stimulated cooperative GTPase activity in solution. Moreover, when Mff was anchored to a lipid template, to mimic a more physiologic environment, significant stimulation of GTPase activity was observed with both WT and ΔVD Drp1. Contrary to recent findings, we show that premature Drp1 self-assembly in solution impairs functional interactions with membrane-anchored Mff. Instead, dimeric Drp1 species are selectively recruited by Mff to initiate assembly of a functional fission complex. Correspondingly, we also found that the coiled-coil motif in Mff is not essential for Drp1 interactions, but rather serves to augment cooperative self-assembly of Drp1 proximal to the membrane. Taken together, our findings provide a mechanism wherein the multimeric states of both Mff and Drp1 regulate their collaborative interaction. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.


    Ryan W Clinton, Christopher A Francy, Rajesh Ramachandran, Xin Qi, Jason A Mears. Dynamin-related Protein 1 Oligomerization in Solution Impairs Functional Interactions with Membrane-anchored Mitochondrial Fission Factor. The Journal of biological chemistry. 2016 Jan 01;291(1):478-92

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 26578514

    View Full Text