Sequence-specific 1H, 13C and 15N backbone resonance assignments of the plakin repeat domain of human envoplakin.

Mark Jeeves, Claudia Fogl, Caezar Al-Jassar, Martyn Chidgey, Michael Overduin

Biomolecular NMR assignments 2016 Apr

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The plakin repeat domain is a distinctive hallmark of the plakin superfamily of proteins, which are found within all epithelial tissues. Plakin repeat domains mediate the interactions of these proteins with the cell cytoskeleton and are critical for the maintenance of tissue integrity. Despite their biological importance, no solution state resonance assignments are available for any homologue. Here we report the essentially complete (1)H, (13)C and (15)N backbone chemical shift assignments of the singular 22 kDa plakin repeat domain of human envoplakin, providing the means to investigate its interactions with ligands including intermediate filaments.

Citation

Mark Jeeves, Claudia Fogl, Caezar Al-Jassar, Martyn Chidgey, Michael Overduin. Sequence-specific 1H, 13C and 15N backbone resonance assignments of the plakin repeat domain of human envoplakin. Biomolecular NMR assignments. 2016 Apr;10(1):167-70