Myung-Ha Song, Ye-Rin Kim, Jun-Won Lee, Chang-Hun Lee, Sang-Yull Lee
International journal of oncology 2016 FebThe cancer/testis antigen NY-SAR-35 is aberrantly expressed in various cancer tissues and cancer cell lines but not in normal tissues except for the testis. A previous study demonstrated that the expression of NY-SAR-35 is activated by hypomethylation in cancer cells. However, the functions of this antigen remain unexplored. In the present study, we investigated the role of NY-SAR‑35 in human embryonic kidney (HEK) 293 cells using exogenous expression system of the gene. NY-SAR‑35 was predominantly expressed at the cytoplasm and was mainly observed in spermatogonia and spermatocytes. Expression of NY-SAR-35 in stable HEK293 transfectant clones was 2-fold higher than the control cells promoting cell growth and proliferation. NY-SAR-35 overexpression also enhanced cell migration and invasion ~2-fold and 4-fold more than the control, respectively. In contrast, small interfering RNA-mediated knockdown of NY-SAR-35 suppressed cell proliferation, migration, and invasion in HEK293 stable transfectants. We concluded that NY-SAR-35 as a cancer/testis antigen enhanced cell proliferation and invasion.
Myung-Ha Song, Ye-Rin Kim, Jun-Won Lee, Chang-Hun Lee, Sang-Yull Lee. Cancer/testis antigen NY-SAR-35 enhances cell proliferation, migration, and invasion. International journal of oncology. 2016 Feb;48(2):569-76
PMID: 26648093
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