Correlation Engine 2.0
Clear Search sequence regions

  • AKL1 (1)
  • ATP (1)
  • CAN1 (1)
  • cells (1)
  • CUE5 (1)
  • dna (1)
  • doxorubicin (10)
  • gene fungal (1)
  • genes (8)
  • PDR5 (4)
  • yeast (1)
  • YHR177W (1)
  • Sizes of these terms reflect their relevance to your search.

    Doxorubicin is one of the most potent anticancer drugs used in the treatment of various cancer types. The efficacy of doxorubicin is influenced by the drug resistance mechanisms and its cytotoxicity. In this study, we performed a high-copy screening analysis to find genes that play a role in doxorubicin resistance and found several genes (CUE5, AKL1, CAN1, YHR177W and PDR5) that provide resistance. Among these genes, overexpression of PDR5 provided a remarkable resistance, and deletion of it significantly rendered the tolerance level for the drug. Q-PCR analyses suggested that transcriptional regulation of these genes was not dependent on doxorubicin treatment. Additionally, we profiled the global expression pattern of cells in response to doxorubicin treatment and highlighted the genes and pathways that are important in doxorubicin tolerance/toxicity. Our results suggest that many efflux pumps and DNA metabolism genes are upregulated by the drug and required for doxorubicin tolerance.


    Ayse Banu Demir, Ahmet Koc. High-Copy Overexpression Screening Reveals PDR5 as the Main Doxorubicin Resistance Gene in Yeast. PloS one. 2015;10(12):e0145108

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 26690737

    View Full Text