A novel bipartite UNC-101/AP-1 μ1 binding signal mediates KVS-4/Kv2.1 somatodendritic distribution in Caenorhabditis elegans.

Xin Zhou, Jia Zeng, Chenxi Ouyang, Qianyun Luo, Miao Yu, Zhenrong Yang, Hui Wang, Kang Shen, Anbing Shi

FEBS letters 2016 Jan

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Potassium channels such as Kv2.1 are targeted to specific subcellular compartments to fulfill various functions. However, the mechanisms for their localization are poorly understood. Here, we show that KVS-4/Kv2.1 somatodendritic localization in Caenorhabditis elegansDA9 neuron requires UNC-101(AP-1 μ subunit). We define a bipartite sorting signal within KVS-4 consisting of a C-terminal EQMIL and N-terminal WNIIE motifs. The bipartite signal is sufficient to target nonpolarized transmembrane protein MIG-13 into DA9 somatodendritic compartments. Furthermore, we found that AP-1 interacts with the bipartite signal through UNC-101/AP-1 μ N-terminal predicted Longin-like domain. Our results provide new insight into the mechanisms of Kv2.1 post-Golgi sorting and targeting. © 2015 Federation of European Biochemical Societies.

Citation

Xin Zhou, Jia Zeng, Chenxi Ouyang, Qianyun Luo, Miao Yu, Zhenrong Yang, Hui Wang, Kang Shen, Anbing Shi. A novel bipartite UNC-101/AP-1 μ1 binding signal mediates KVS-4/Kv2.1 somatodendritic distribution in Caenorhabditis elegans. FEBS letters. 2016 Jan;590(1):76-92