Ki-67 antigen expression in the mammary epithelium of female rats in persistent estrus treated with raloxifene.

G V de Sousa, C S Borges, P V Lopes-Costa, D R Costa-Silva, S F Lopes-Dias, A C P Nazário, A P Alencar, B B da Silva

European journal of gynaecological oncology 2015

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To evaluate Ki-67 antigen expression in the mammary epithelium of female rats in persistent estrus treated with raloxifene. Forty-one Wistar-Hannover rats in persistent estrus induced by 1.25 mg of testosterone propionate were randomly divided into two groups: Group A (control, n = 21) in which the animals received only the vehicle (propylene glycol) and Group B (experimental, n = 20) in which the rats received 750 µg/day of raloxifene by gavage. After 21 days of treatment, all the animals were sacrificed and the first pair of abdominal-inguinal mammary glands was extirpated and fixed in 10% buffered formalin to investigate Ki-67 expression by immunohistochemistry. The data were analysed using Student's t-test (p < 0.05). The percentage of Ki-67-stained nuclei per 500 cells in the mammary epithelium was 42.33 ± 6.18 and 15.51 ± 3.71 [mean ± standard error of the mean (SEM)] in the control and experimental groups, respectively (p < 0.001). Raloxifene treatment significantly reduced Ki-67 expression in the mammary epithelium of rats in persistent estrus.

Citation

G V de Sousa, C S Borges, P V Lopes-Costa, D R Costa-Silva, S F Lopes-Dias, A C P Nazário, A P Alencar, B B da Silva. Ki-67 antigen expression in the mammary epithelium of female rats in persistent estrus treated with raloxifene. European journal of gynaecological oncology. 2015;36(6):694-7