Julio A Landero Figueroa, Cory A Stiner, Tatiana L Radzyukevich, Judith A Heiny
Scientific reports 2016The use of ICP-MS to measure metal ion content in biological tissues offers a highly sensitive means to study metal-dependent physiological processes. Here we describe the application of ICP-MS to measure membrane transport of Rb and K ions by the Na,K-ATPase in mouse skeletal muscles and human red blood cells. The ICP-MS method provides greater precision and statistical power than possible with conventional tracer flux methods. The method is widely applicable to studies of other metal ion transporters and metal-dependent processes in a range of cell types and conditions.
Julio A Landero Figueroa, Cory A Stiner, Tatiana L Radzyukevich, Judith A Heiny. Metal ion transport quantified by ICP-MS in intact cells. Scientific reports. 2016;6:20551
PMID: 26838181
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