Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells.

Alexey N Butkevich, Gyuzel Yu Mitronova, Sven C Sidenstein, Jessica L Klocke, Dirk Kamin, Dirk N H Meineke, Elisa D'Este, Philip-Tobias Kraemer, Johann G Danzl, Vladimir N Belov, Stefan W Hell

Angewandte Chemie (International ed. in English) 2016 Mar 01

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A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm. © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

Citation

Alexey N Butkevich, Gyuzel Yu Mitronova, Sven C Sidenstein, Jessica L Klocke, Dirk Kamin, Dirk N H Meineke, Elisa D'Este, Philip-Tobias Kraemer, Johann G Danzl, Vladimir N Belov, Stefan W Hell. Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells. Angewandte Chemie (International ed. in English). 2016 Mar 01;55(10):3290-4