Correlation Engine 2.0
Clear Search sequence regions

  • Annexin V (1)
  • band (3)
  • cell (6)
  • fibroblasts (3)
  • glyoxal (3)
  • light (1)
  • membrane (3)
  • mice (1)
  • mitochondria (2)
  • propidium (1)
  • resazurin (1)
  • skin lesions (1)
  • vitro (1)
  • yo pro- 1 (1)
  • Sizes of these terms reflect their relevance to your search.

    Exposures of the skin with electromagnetic radiation of wavelengths between 670 nm and 1400 nm are often used as a general treatment to improve wound healing and reduce pain, for example, in chronic diabetic skin lesions. We investigated the effects of water-filtered infrared A (wIRA) and of narrow-band IR-A provided by a light-emitting diode LED (LED-IR-A) irradiation in vitro on 3T3 fibroblast cultures under defined conditions with and without glyoxal administration. Glyoxal triggers the formation of advanced glycation end products, thereby mimicking a diabetic metabolic state. Cell viability and apoptotic changes were determined by flow cytometry after vital staining with Annexin V, YO-PRO-1 and propidium iodide (PI), and by SubG1 assay. Mitochondrial function and oxidative stress were examined by vital staining for radical production, mitochondrial membrane potential (MMP) and the ratio of reduced-to-oxidized glutathione (GSH/GSSG). The metabolic state was monitored by a resazurin conversion assay. The numbers of apoptotic cells were reduced in cultures irradiated with wIRA or LED-IR-A. More mitochondria showed a well-polarized MMP after wIRA irradiation in glyoxal damaged cells. LED-IR-A treatment specifically restored the GSH/GSSG ratio. The immediate positive effects of wIRA and LED-IR-A observed in living cells, particularly on mitochondria, reflect the therapeutic benefits of wIRA and LED-IR-A. © 2016 The American Society of Photobiology.


    Lilla Knels, Monika Valtink, Helmut Piazena, Jamlec de la Vega Marin, Kerstin Gommel, Amelie Lupp, Cora Roehlecke, Mirko Mehner, Richard H W Funk. Effects of Narrow-band IR-A and of Water-Filtered Infrared A on Fibroblasts. Photochemistry and photobiology. 2016 May;92(3):475-87

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 26876482

    View Full Text