A critical role for the protein kinase PKK in the maintenance of recirculating mature B cells and the development of B1 cells.

Protein kinase C associated kinase (PKK) regulates NF-κB activation and is required for the survival of certain lymphoma cells. Mice lacking PKK die soon after birth, and previous studies suggest that the role of PKK in B cell development might be context dependent. We have generated a mouse strain harboring conditional null alleles for PKK and a Cre-recombinase transgene under the control of the endogenous CD19 promoter. In the present study, we show that knockout of PKK in B cells results in the reduction of long-lived recirculating mature B cell population in lymph nodes and bone marrow as well as a decrease in peritoneal B1 cells, while PKK deficiency has no apparent effect on early B cell development in bone marrow or the development of follicular and marginal zone B cells in the spleen. In addition, we demonstrate that PKK-deficient B cells display defective proliferation and survival responses to stimulation of B cell receptor (BCR), which may underlie the reduction of recirculating mature B cells in PKK mutant mice. Consistently, BCR-mediated NF-κB activation, known to be required for the survival of activated but not resting B cells, is attenuated in PKK-deficient B cells. Thus, our results reveal a critical role of PKK in the maintenance of recirculating mature B cells as well as the development of B1 cells in mice. Copyright © 2016 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

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Luojing Chen, David Oleksyn, Mary Pulvino, Ignacio Sanz, Daniel Ryan, Charlotte Ryan, Chyuan-Sheng Lin, Brian Poligone, Alice P Pentland, Christopher Ritchlin, Jiyong Zhao. A critical role for the protein kinase PKK in the maintenance of recirculating mature B cells and the development of B1 cells. Immunology letters. 2016 Apr;172:67-78

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PMID: 26921474

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