Overexpression of FKS1 to improve yeast autolysis-stress].

With the development of high gravity brewing, yeast cells are exposed to multiple brewing-associated stresses, such as increased osmotic pressure, enhanced alcohol concentration and nutritional imbalance. These will speed up yeast autolysis, which seriously influence beer flavor and quality. To increase yeast anti-autolytic ability, FKS1 overexpression strain was constructed by 18S rDNA. The concentration of β-1,3-glucan of overexpression strain was 62% higher than that of wild type strain. Meantime, FKS1 overexpression strain increased anti-stress ability at 8% ethanol, 0.4 mol/L NaCl and starvation stress. Under simulated autolysis, FKS1 showed good anti-autolytic ability by slower autolysis. These results confirms the potential of FKS1 overexpression to tackle yeast autolysis in high-gravity brewing.

Citation

Jia Li, Jinjing Wang, Qi Li. Overexpression of FKS1 to improve yeast autolysis-stress]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 2015 Sep;31(9):1344-54

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PMID: 26955712

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