Generation and Standardized, Systemic Phenotypic Analysis of Pou3f3L423P Mutant Mice.

Increased levels of blood plasma urea were used as phenotypic parameter for establishing novel mouse models for kidney diseases on the genetic background of C3H inbred mice in the phenotype-driven Munich ENU mouse mutagenesis project. The phenotypically recessive mutant line HST011 was established and further analyzed. The causative mutation was detected in the POU domain, class 3 transcription factor 3 (Pou3f3) gene, which leads to the amino acid exchange Pou3f3L423P thereby affecting the conserved homeobox domain of the protein. Pou3f3 homozygous knockout mice are published and show perinatal death. Line Pou3f3L423P is a viable mouse model harboring a homozygous Pou3f3 mutation. Standardized, systemic phenotypic analysis of homozygous mutants was carried out in the German Mouse Clinic. Main phenotypic changes were low body weight and a state of low energy stores, kidney dysfunction and secondary effects thereof including low bone mineralization, multiple behavioral and neurological defects including locomotor, vestibular, auditory and nociceptive impairments, as well as multiple subtle changes in immunological parameters. Genome-wide transcriptome profiling analysis of kidney and brain of Pou3f3L423P homozygous mutants identified significantly regulated genes as compared to wild-type controls.

Citation

Sudhir Kumar, Birgit Rathkolb, Elisabeth Kemter, Sibylle Sabrautzki, Dian Michel, Thure Adler, Lore Becker, Johannes Beckers, Dirk H Busch, Lillian Garrett, Wolfgang Hans, Sabine M Hölter, Marion Horsch, Martin Klingenspor, Thomas Klopstock, Ildikó Rácz, Jan Rozman, Ingrid Liliana Vargas Panesso, Alexandra Vernaleken, Andreas Zimmer, Helmut Fuchs, Valérie Gailus-Durner, Martin Hrabě de Angelis, Eckhard Wolf, Bernhard Aigner. Generation and Standardized, Systemic Phenotypic Analysis of Pou3f3L423P Mutant Mice. PloS one. 2016;11(3):e0150472

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PMID: 27003440

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