Supramolecular organization of the human N-BAR domain in shaping the sarcolemma membrane.

The 30kDa N-BAR domain of the human Bin1 protein is essential for the generation of skeletal muscle T-tubules. By electron cryo-microscopy and electron cryo-tomography with a direct electron detector, we found that Bin1-N-BAR domains assemble into scaffolds of low long-range order that form flexible membrane tubules. The diameter of the tubules closely matches the curved shape of the N-BAR domain, which depends on the composition of the target membrane. These insights are fundamental to our understanding of T-tubule formation and function in human skeletal muscle. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Bertram Daum, Andrea Auerswald, Tobias Gruber, Gerd Hause, Jochen Balbach, Werner Kühlbrandt, Annette Meister. Supramolecular organization of the human N-BAR domain in shaping the sarcolemma membrane. Journal of structural biology. 2016 Jun;194(3):375-82

PMID: 27016283

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