A simplified method for the preparation of WGA-Sepharose 4B and its use in the purification of MN blood group antigens.
S M Zhang, J X Li, Y M Wei, Z M Yang
Department of Biochemistry, Second Military Medical College, Shanghai, People's Republic of China.
Biomedical chromatography : BMC 1989 JanA simplified method for the preparation of wheat germ agglutinin(WGA)-Sepharose 4B by coupling highly purified WGA, prepared by improved affinity chromatography, with BrCN activated Sepharose 4B in a solution of high carbonate buffer is described. The amount of WGA linked to Sepharose 4B was 82.40% (3.07 mg WGA per ml Sepharose 4B). MN blood group antigens of human erythrocyte membranes purified with WGA-Sepharose 4B affinity chromatography showed a single band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE). The yield of the antigens from 400 mL fresh blood was 32-40 mg. The WGA-Sepharose 4B column could be used several times without loss of activity.
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S M Zhang, J X Li, Y M Wei, Z M Yang. A simplified method for the preparation of WGA-Sepharose 4B and its use in the purification of MN blood group antigens. Biomedical chromatography : BMC. 1989 Jan;3(1):10-3
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PMID: 2706359
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