Eduardo Gómez-Conde, Miguel Ángel Vargas-Mejía, María Alicia Díaz-Orea, Rosaura Hernández-Rivas, María Elena Cárdenas-Perea, Tayde Guerrero-González, Juan Antonio González-Barrios, Álvaro José Montiel-Jarquín
Experimental parasitology 2016 AugIt is known that the microtubules (MT) of Entamoeba histolytica trophozoites form an intranuclear mitotic spindle. However, electron microscopy studies and the employment of anti-beta-tubulin (β-tubulin) antibodies have not exhibited these cytoskeletal structures in the cytoplasm of these parasites. The purpose of this work was to detect β-tubulin in the cytoplasm of interphasic E. histolytica trophozoites. Activated or non-activated HMI-IMSS-strain E. histolytica trophozoites were used and cultured for 72 h at 37 °C in TYI-S-33 medium, and then these were incubated with the anti-β-tubulin antibody of E. histolytica. The anti-β-tubulin antibody reacted with the intranuclear mitotic spindle of E. histolytica-activated trophozoites as control. In contrast, in non-activated interphasic parasites, anti-β-tubulin antibody reacted with diverse puntiform structures in the cytoplasm and with ring-shaped structures localized in the cytoplasm, cellular membrane and endocytic stomas. In this work, for the first time, the presence of β-tubulin is shown in the cytoplasm of E. histolytica trophozoites. Copyright © 2016 Elsevier Inc. All rights reserved.
Eduardo Gómez-Conde, Miguel Ángel Vargas-Mejía, María Alicia Díaz-Orea, Rosaura Hernández-Rivas, María Elena Cárdenas-Perea, Tayde Guerrero-González, Juan Antonio González-Barrios, Álvaro José Montiel-Jarquín. Detection of beta-tubulin in the cytoplasm of the interphasic Entamoeba histolytica trophozoites. Experimental parasitology. 2016 Aug;167:38-42
PMID: 27156446
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