Magdaléna Röderová, Miroslava Htoutou Sedláková, Vendula Pudová, Kristýna Hricová, Romana Silová, Peter Eghonghon Odion Imwensi, Jan Bardoň, Milan Kolář
The new microbiologica 2016 AprThe aims were to investigate the level of antibiotic-resistant bacteria in hospital and urban wastewater and to determine the similarity of isolates obtained from wastewater and hospitalized patients. Wastewater samples were collected in September 2013 and 2014. After identification using MALDI-TOF MS, beta-lactamase production was determined by relevant phenotypic tests. Genes responsible for the production of single beta-lactamase groups and Qnr proteins were established. The epidemiological relationship of the isolates from wastewater and hospitalized patients was determined by PFGE. A total of 51 isolates of enterobacteria were obtained. Overall, 45.1% of them produced broad-spectrum beta-lactamases. Genes encoding TEM, SHV, CTX-M, CIT, DHA and EBC types of enzymes and Qnr proteins were detected. No broad-spectrum beta-lactamase production was confirmed in the urban wastewater treatment plant. The most important finding was the detection of two identical isolates of K. pneumoniae in 2013, one from a patient's urinary catheter and the other from a wastewater sample.
Magdaléna Röderová, Miroslava Htoutou Sedláková, Vendula Pudová, Kristýna Hricová, Romana Silová, Peter Eghonghon Odion Imwensi, Jan Bardoň, Milan Kolář. Occurrence of bacteria producing broad-spectrum beta-lactamases and qnr genes in hospital and urban wastewater samples. The new microbiologica. 2016 Apr;39(2):124-33
PMID: 27196551
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