Structural and functional studies of a large winged Z-DNA-binding domain of Danio rerio protein kinase PKZ.

Vinod Kumar Subramani, Doyoun Kim, Kyunghee Yun, Kyeong Kyu Kim

FEBS letters 2016 Jul

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The Z-DNA-binding domain of PKZ from zebrafish (Danio rerio; drZαPKZ ) contains the largest β-wing among known Z-DNA-binding domains. To elucidate the functional implication of the β-wing, we solved the crystal structure of apo-drZαPKZ . Structural comparison with its Z-DNA-bound form revealed a large conformational change within the β-wing during Z-DNA binding. Biochemical studies of protein mutants revealed that two basic residues in the β-wing are responsible for Z-DNA recognition as well as fast B-Z transition. Therefore, the extra basic residues in the β-wing of drZαPKZ are necessary for the fast B-Z transition activity. © 2016 Federation of European Biochemical Societies.

Citation

Vinod Kumar Subramani, Doyoun Kim, Kyunghee Yun, Kyeong Kyu Kim. Structural and functional studies of a large winged Z-DNA-binding domain of Danio rerio protein kinase PKZ. FEBS letters. 2016 Jul;590(14):2275-85