Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.

Yuri L Lyubchenko, Luda S Shlyakhtenko

Critical reviews in eukaryotic gene expression 2016

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This article reviews atomic force microscopy (AFM) studies of DNA structure and dynamics and protein-DNA complexes, including recent advances in the visualization of protein-DNA complexes with the use of cutting-edge, high-speed AFM. Special emphasis is given to direct nanoscale visualization of dynamics of protein-DNA complexes. In the area of DNA structure and dynamics, structural studies of local non-B conformations of DNA and the interplay of local and global DNA conformations are reviewed. The application of time-lapse AFM nanoscale imaging of DNA dynamics is illustrated by studies of Holliday junction branch migration. Structure and dynamics of protein-DNA interactions include problems related to site-specific DNA recombination, DNA replication, and DNA mismatch repair. Studies involving the structure and dynamics of chromatin are also described.

Citation

Yuri L Lyubchenko, Luda S Shlyakhtenko. Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy. Critical reviews in eukaryotic gene expression. 2016;26(1):63-96