Central loop of non-conventional toxin WTX from Naja kaouthia is important for interaction with nicotinic acetylcholine receptors.

Three-finger' toxin WTX from Naja kaouthia interacts with nicotinic and muscarinic acetylcholine receptors (nAChRs and mAChRs). Mutagenesis and competition experiments with (125)I-α-bungarotoxin revealed that Arg31 and Arg32 residues from the WTX loop II are important for binding to Torpedo californica and human α7 nAChRs. Computer modeling suggested that loop II occupies the orthosteric binding site at α7 nAChR. The similar toxin interface was previously described as a major determinant of allosteric interactions with mAChRs. Copyright © 2016 Elsevier Ltd. All rights reserved.

Citation

Ekaterina N Lyukmanova, Mikhail A Shulepko, Zakhar O Shenkarev, Igor E Kasheverov, Anton O Chugunov, Dmitrii S Kulbatskii, Mikhail Yu Myshkin, Yuri N Utkin, Roman G Efremov, Victor I Tsetlin, Alexander S Arseniev, Mikhail P Kirpichnikov, Dmitry A Dolgikh. Central loop of non-conventional toxin WTX from Naja kaouthia is important for interaction with nicotinic acetylcholine receptors. Toxicon : official journal of the International Society on Toxinology. 2016 Sep 1;119:274-9

PMID: 27343701

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