Immunoproteomic studies on paediatric opsoclonus-myoclonus associated with neuroblastoma.

We aimed to identify new cell-membrane antigens implicated in opsoclonus-myoclonus with neuroblastoma. The sera of 3 out of 14 patients showed IgG electron-microscopy immunogold reactivity on SH-SY5Y neuroblastoma cells. Immunoprecipitation experiments using rat brain synaptosomes and SH-SY5Y cells led to the identification of: (1) thirty-one nuclear/cytoplasmic proteins (including antigens HuB, HuC); (2) seven neuronal membrane proteins, including the Shaw-potassium channel Kv3.3 (KCNC3), whose genetic disruption in mice causes ataxia and generalized muscle twitching. Although cell-based assays did not demonstrate direct antigenicity, our findings point to Shaw-related subfamily of the potassium voltage-gated channels complexed proteins as hypothetical antigenic targets. Copyright © 2016 Elsevier B.V. All rights reserved.

Citation

Estefanía Torres-Vega, María Durán-Moreno, Manuel Sánchez Del Pino, Yania Yáñez, Adela Cañete, Victoria Castel, Rogelio López-Cuevas, Juan Jesús Vílchez, Josep Dalmau, Francesc Graus, José Manuel García Verdugo, Luis Bataller. Immunoproteomic studies on paediatric opsoclonus-myoclonus associated with neuroblastoma. Journal of neuroimmunology. 2016 Aug 15;297:98-102

PMID: 27397082

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