Tetraspanin 7 regulates sealing zone formation and the bone-resorbing activity of osteoclasts.

Jun-Oh Kwon, Yong Deok Lee, Haemin Kim, Min Kyung Kim, Min-Kyoung Song, Zang Hee Lee, Hong-Hee Kim

Biochemical and biophysical research communications 2016 Sep 02

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Tetraspanin family proteins regulate morphology, motility, fusion, and signaling in various cell types. We investigated the role of the tetraspanin 7 (Tspan7) isoform in the differentiation and function of osteoclasts. Tspan7 was up-regulated during osteoclastogenesis. When Tspan7 expression was reduced in primary precursor cells by siRNA-mediated gene knock-down, the generation of multinuclear osteoclasts was not affected. However, a striking cytoskeletal abnormality was observed: the formation of the podosome belt structure was inhibited and the microtubular network were disrupted by Tspan7 knock-down. Decreases in acetylated microtubules and levels of phosphorylated Src and Pyk2 in Tspan7 knock-down cells supported the involvement of Tspan7 in cytoskeletal rearrangement signaling in osteoclasts. This cytoskeletal defect interfered with sealing zone formation and subsequently the bone-resorbing activity of mature osteoclasts on dentin surfaces. Our results suggest that Tspan7 plays an important role in cytoskeletal organization required for the bone-resorbing function of osteoclasts by regulating signaling to Src, Pyk2, and microtubules. Copyright © 2016 Elsevier Inc. All rights reserved.

Citation

Jun-Oh Kwon, Yong Deok Lee, Haemin Kim, Min Kyung Kim, Min-Kyoung Song, Zang Hee Lee, Hong-Hee Kim. Tetraspanin 7 regulates sealing zone formation and the bone-resorbing activity of osteoclasts. Biochemical and biophysical research communications. 2016 Sep 02;477(4):1078-1084