Tensin 3 is a new partner of Dock5 that controls osteoclast podosome organization and activity.

Bone resorption by osteoclasts is mediated by a typical adhesion structure called the sealing zone or actin ring, whose architecture is based on a belt of podosomes. The molecular mechanisms driving podosome organization into superstructures remain poorly understood to date, in particular at the osteoclast podosome belt. We performed proteomic analyses in osteoclasts and found that the adaptor protein tensin 3 is a partner of Dock5, a Rac exchange factor necessary for podosome belt formation and bone resorption. Expression of tensin 3 and Dock5 concomitantly increase during osteoclast differentiation. These proteins associate with the osteoclast podosome belt but not with individual podosomes, in contrast to vinculin. Super-resolution microscopy revealed that, even if they colocalize in the x-y plane of the podosome belt, Dock5 and tensin 3 differentially localize relative to vinculin in the z-axis. Tensin 3 increases Dock5 exchange activity towards Rac, and suppression of tensin 3 in osteoclasts destabilizes podosome organization, leading to delocalization of Dock5 and a severe reduction in osteoclast activity. Our results suggest that Dock5 and tensin 3 cooperate for osteoclast activity, to ensure the correct organization of podosomes. © 2016. Published by The Company of Biologists Ltd.

Citation

Heiani Touaitahuata, Anne Morel, Serge Urbach, Julio Mateos-Langerak, Sylvain de Rossi, Anne Blangy. Tensin 3 is a new partner of Dock5 that controls osteoclast podosome organization and activity. Journal of cell science. 2016 Sep 15;129(18):3449-61

PMID: 27505886

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