Single-molecule fluorescence microscopy of native macromolecular complexes.

Macromolecular complexes consisting of proteins, lipids, and/or nucleic acids are ubiquitous in biological processes. Their composition, stoichiometry, order of assembly, and conformations can be heterogeneous or can change dynamically, making single-molecule studies best suited to measure these properties accurately. Recent single-molecule pull-down and other related approaches have combined the principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native macromolecular complexes. In this review, we present the advances in single-molecule pull-down methods and biological systems that have been investigated in such semi vivo manner. Copyright © 2016 Elsevier Ltd. All rights reserved.

Citation

Vasudha Aggarwal, Taekjip Ha. Single-molecule fluorescence microscopy of native macromolecular complexes. Current opinion in structural biology. 2016 Dec;41:225-232

PMID: 27662375

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