A novel trichosanthin fusion protein with increased cytotoxicity to tumor cells.

Bing Lin, Xu-Zhong Yang, Xue-Wei Cao, Tao-Zhu Zhang, Fu-Jun Wang, Jian Zhao

Biotechnology letters 2017 Jan

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To evaluate the anti-tumor effects of trichosanthin after fusion with a cell penetrating peptide, heparin-binding peptide (HBP), derived from human heparin-binding EGF-like growth factor (HB-EGF). The fusion protein of trichosanthin-HBP was expressed in Escherichia coli BL21 and purified by Ni-NTA affinity chromatography. The HBP domain had no influence on the topological inactivation activity and N-glycosidase activity of trichosanthin. Trichosanthin-HBP significantly inhibited the growth of tested cancer cells which are impervious to trichosanthin. Tumor cell apoptosis and both the mitochondrial- and death receptor-mediated apoptotic signaling pathways induced by trichosanthin-HBP were more significant than those induced by trichosanthin in HeLa cells. HBP is an efficient intracellular delivery vehicle for trichosanthin and makes trichosanthin-HBP become a promising agent for cancer therapy.

Citation

Bing Lin, Xu-Zhong Yang, Xue-Wei Cao, Tao-Zhu Zhang, Fu-Jun Wang, Jian Zhao. A novel trichosanthin fusion protein with increased cytotoxicity to tumor cells. Biotechnology letters. 2017 Jan;39(1):71-78