eis Promoter C14G and C15G Mutations Do Not Confer Kanamycin Resistance in Mycobacterium tuberculosis.

Suporn Pholwat, Suzanne Stroup, Scott Heysell, Oleg Ogarkov, Svetlana Zhdanova, Girija Ramakrishnan, Eric Houpt

Antimicrobial agents and chemotherapy 2016 Dec

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We studied the significance of particular eis mutations on Mycobacterium tuberculosis drug resistance using a specialized transduction strategy. Recombinant strains harboring eis promoter mutations C14T, C12T, and G10A exhibited kanamycin resistance with MICs of 40, 10, and 20 μg/ml, respectively, while recombinant strains harboring C14G and C15G mutations were kanamycin susceptible (MIC, 2.5 to 5 μg/ml). Each of the eis mutants tested remained amikacin susceptible (MIC, 0.5 to 4 μg/ml). The identification of specific eis mutations is needed for accurate genotypic susceptibility testing for kanamycin. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Citation

Suporn Pholwat, Suzanne Stroup, Scott Heysell, Oleg Ogarkov, Svetlana Zhdanova, Girija Ramakrishnan, Eric Houpt. eis Promoter C14G and C15G Mutations Do Not Confer Kanamycin Resistance in Mycobacterium tuberculosis. Antimicrobial agents and chemotherapy. 2016 Dec;60(12):7522-7523