Production of uracil from methane by a newly isolated Methylomonas sp. SW1.

Sangwoo Kim, Wangjun Lee, Insu Song, Yuhyun Kwon, Seokhun Yun, Soohyun Park, Sukhyeong Cho, Byung-Keun Oh, Han Bin Oh, Jinwon Lee

Journal of biotechnology 2016 Dec 20

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Methane is an abundant, inexpensive one-carbon feedstock and one of the most powerful greenhouse gases. Because it does not compete with food demand, it is considered a promising carbon feedstock for the production of valuable products using methanotrophic bacteria. Here, we isolated a novel methanotrophic bacterium, Methylomonas sp. SW1, from a sewage sample obtained from Wonju City Water Supply Drainage Center, Republic of Korea. The conditions for uracil production by Methylomonas sp. SW1, such as Cu2+ concentration and temperature were investigated and optimized. As a result, Methylomonas sp. SW1 produced uracil from methane as a sole carbon source with a titer of 2.1mg/L in 84h without genetic engineering under the optimized condition. The results in this study demonstrate the feasibility of using Methylomonas sp. SW1 for the production of uracil from methane. This is the first report of uracil production from gas feedstock by methanotrophic bacteria. Copyright © 2016 Elsevier B.V. All rights reserved.

Citation

Sangwoo Kim, Wangjun Lee, Insu Song, Yuhyun Kwon, Seokhun Yun, Soohyun Park, Sukhyeong Cho, Byung-Keun Oh, Han Bin Oh, Jinwon Lee. Production of uracil from methane by a newly isolated Methylomonas sp. SW1. Journal of biotechnology. 2016 Dec 20;240:43-47