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In vitro strategies to obtain different T helper cell subsets from naïve precursors are usually characterized by anti-CD3 plus anti-CD28 monoclonal antibody stimulation of naïve CD4+ T cells (derived from both peripheral blood or umbilical cord blood) in the presence of specific polarizing cytokines. In this chapter, we describe detailed protocols to obtain in vitro differentiation of human naïve CD4+ T cells into Th1, Th2, or Th17 cells.


Francesco Annunziato, Laura Maggi. Strategies for T Helper Cell Subset Differentiation from Naïve Precursors. Methods in molecular biology (Clifton, N.J.). 2017;1514:127-137

PMID: 27787798

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