Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens.

Kazuya Suzuki, Keisuke Oguma, Hiroshi Sentsui

The Journal of veterinary medical science 2017 Jan 20

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We attempted to prepare a cell line that produces maedi/visna virus (MVV) and is free of contamination by other viruses and mycoplasmas. Three cell lines, which originated from a sheep, goat and bat, were infected with MVV and passaged approximately every 5 days. The cultured cells were then subjected to polymerase chain reaction analysis for MVV provirus. As a result, a cell line persistently infected with MVV was established from ZZ-R cells, which originated from the fetal goat tongue. The 50-fold concentrated culture fluid formed a precipitation line against reference antiserum.

Citation

Kazuya Suzuki, Keisuke Oguma, Hiroshi Sentsui. Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens. The Journal of veterinary medical science. 2017 Jan 20;79(1):141-143