A Morphological identification cell cytotoxicity assay using cytoplasm-localized fluorescent probe (CLFP) to distinguish living and dead cells.

Fangfang Lai, Zhengwei Shen, Hui Wen, Jialing Chen, Xiang Zhang, Ping Lin, Dali Yin, Huaqing Cui, Xiaoguang Chen

Biochemical and biophysical research communications 2017 Jan 08

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Cell cytotoxicity assays include cell activity assays and morphological identification assays. Currently, all frequently used cytotoxicity assays belong to cell activity assays but suffer from detection limitations. Morphological identification of cell death remains as the gold standard, although the method is difficult to scale up. At present there is no generally accepted morphological identification based cell cytotoxicity assay. In this study, we applied previous developed cell cytoplasm-localized fluorescent probe (CLFP) to display cell morphologies. Under fluorescence microscopy, the fluorescence morphology and intensity of living cells are distinct from dead cells. Based on these characters we extracted the images of living cells from series of samples via computational analysis. Thus, a novel cell morphological identification cytotoxicity assay (CLFP assay) is developed. The performance of the CLFP assay was similar to cell activity assay (MTT assay), but the accuracy of the CLFP assay was superior when measuring the cytotoxicity of active compounds. Copyright © 2016 Elsevier Inc. All rights reserved.

Citation

Fangfang Lai, Zhengwei Shen, Hui Wen, Jialing Chen, Xiang Zhang, Ping Lin, Dali Yin, Huaqing Cui, Xiaoguang Chen. A Morphological identification cell cytotoxicity assay using cytoplasm-localized fluorescent probe (CLFP) to distinguish living and dead cells. Biochemical and biophysical research communications. 2017 Jan 08;482(2):257-263