Effect of CPNE7 siRNA on cell proliferation and osteogenic differentiation in human periodontal ligament cells].

Cai-Hong Liu, Hai-Yan Hu

Shanghai kou qiang yi xue = Shanghai journal of stomatology 2016 Aug

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To investigate the effect and mechanism of CPNE7 siRNA on proliferation and osteogenic differentiation in human periodontal ligament cells (hPDLs). hPDLs were isolated by enzyme digestion, transfected with pSUPER-CPNE7 in order to knock down CPNE7. The expression of CPNE7 mRNA and protein was detected by Western blot and RT-PCR; ELISA was carried out for the activity of NF-κB; hPDLs were pretreated with PDTC (10 μmol/L) for 30 min, CCK8 was used to evaluate the proliferation; ALP activity was assayed with ELISA; The expression of RUNX2, OSX and OCN was measured with RT-PCR and Western blot. The data were analyzed with SPSS11.0 software package. After transfection with pSUPER-CPNE7, CPNE7 expression was significantly decreased (P<0.05). ELISA assay indicated that CPNE7 siRNA enhanced NF-κB activity. CCK8 and RT-PCR assay showed that CPNE7 siRNA inhibited cell proliferation, ALP activity and decreased the expression of RUNX2, OSX and OCN in hPDLs; however, these effects were abolished by PDTC. CPNE7 siRNA inhibits the proliferation and osteogenic differentiation of hPDLs through NF-κB pathway.

Citation

Cai-Hong Liu, Hai-Yan Hu. Effect of CPNE7 siRNA on cell proliferation and osteogenic differentiation in human periodontal ligament cells]. Shanghai kou qiang yi xue = Shanghai journal of stomatology. 2016 Aug;25(4):420-425