Super RLuc8: A novel engineered Renilla luciferase with a red-shifted spectrum and stable light emission.

Somaieh Rahnama, Behnaz Saffar, Zahra Fanaei Kahrani, Mahboobeh Nazari, Rahman Emamzadeh

Enzyme and microbial technology 2017 Jan

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Renilla luciferase is a bioluminescent enzyme which is broadly used as a reporter protein in molecular biosensors. In this study, a novel luciferase with desired light emission wavelength and thermostability is reported. The results indicated that the new luciferase, namely super RLuc8, had a red-shifted spectrum and showed stable light emission. Super RLuc8 showed a 10-fold (p-value=0.0084) increase in the thermostability at 37°C after 20min incubation, in comparison to the native enzyme. The optimum temperature of the mutant increased from 30 to 37°C. Molecular dynamics simulation analysis indicated that the increased thermostability was most probably caused by a better structural compactness and more local rigidity in the regions out of the emitter site. Copyright © 2016 Elsevier Inc. All rights reserved.

Citation

Somaieh Rahnama, Behnaz Saffar, Zahra Fanaei Kahrani, Mahboobeh Nazari, Rahman Emamzadeh. Super RLuc8: A novel engineered Renilla luciferase with a red-shifted spectrum and stable light emission. Enzyme and microbial technology. 2017 Jan;96:60-66