Extraction of high-quality RNA from human articular cartilage.

Heather K Le Bleu, Fadia A Kamal, Meghan Kelly, John P Ketz, Michael J Zuscik, Reyad A Elbarbary

Analytical biochemistry 2017 Feb 01

filter terms:

Extracting high-quality RNA from articular cartilage is challenging due to low cellularity and high proteoglycan content. This problem hinders efficient application of RNA sequencing (RNA-seq) analysis in studying cartilage homeostasis. Here we developed a method that purifies high-quality RNA directly from cartilage. Our method optimized the collection and homogenization steps so as to minimize RNA degradation, and modified the conventional TRIzol protocol to enhance RNA purity. Cartilage RNA purified using our method has appropriate quality for RNA-seq experiments including an RNA integrity number of ∼8. Our method also proved efficient in extracting high-quality RNA from subchondral bone. Copyright © 2016 Elsevier Inc. All rights reserved.

Citation

Heather K Le Bleu, Fadia A Kamal, Meghan Kelly, John P Ketz, Michael J Zuscik, Reyad A Elbarbary. Extraction of high-quality RNA from human articular cartilage. Analytical biochemistry. 2017 Feb 01;518:134-138