Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells.

Atsushi Yagishita, Tasuku Ueno, Hiroyasu Esumi, Hideyuki Saya, Kazuhiro Kaneko, Katsuya Tsuchihara, Yasuteru Urano

Bioconjugate chemistry 2017 Feb 15

filter terms:

Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

Citation

Atsushi Yagishita, Tasuku Ueno, Hiroyasu Esumi, Hideyuki Saya, Kazuhiro Kaneko, Katsuya Tsuchihara, Yasuteru Urano. Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells. Bioconjugate chemistry. 2017 Feb 15;28(2):302-306