Immunocytochemical detection of rhamnogalacturonan II on forming cell plates in cultured tobacco cells.

Rhamnogalacturonan II (RG-II) is a region of pectin macromolecules that is present in plant primary cell walls. The RG-II region serves as the site of borate cross-linking within pectin, via which pectin macromolecules link together to form a gel. In this study, we examined whether RG-II is present in the cell plate, the precursor of primary cell walls that forms during cytokinesis. A structure inside dividing cells was labeled with a rabbit polyclonal anti-RG-II antibody and detected by immunofluorescence microscopy. An antibody against callose, a marker polysaccharide for the cell plate, also labeled the structure. In immunoelectron microscopy analyses using the anti-RG-II antibody, gold particles were distributed in electron-lucent vesicular structures that appeared to correspond to the forming cell plates in late anaphase cells. Together, these results suggest that RG-II is present in cell plates from the early phase of their assembly.

Citation

Ye Zhou, Tatsuya Awano, Masaru Kobayashi, Toru Matoh, Keiji Takabe. Immunocytochemical detection of rhamnogalacturonan II on forming cell plates in cultured tobacco cells. Bioscience, biotechnology, and biochemistry. 2017 May;81(5):899-905

Mesh Tags

Substances

PMID: 28049369

search → result