Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.

Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.

Citation

Georgia K Atkin-Smith, Stephanie Paone, Damien J Zanker, Mubing Duan, Than K Phan, Weisan Chen, Mark D Hulett, Ivan K H Poon. Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting. Scientific reports. 2017 Jan 06;7:39846

PMID: 28057919

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