Preparation of Cell Cultures and Vaccinia Virus Stocks.

Catherine A Cotter, Patricia L Earl, Linda S Wyatt, Bernard Moss

Current protocols in molecular biology 2017 Jan 05

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The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2017 by John Wiley & Sons, Inc. Copyright © 2017 John Wiley & Sons, Inc.

Citation

Catherine A Cotter, Patricia L Earl, Linda S Wyatt, Bernard Moss. Preparation of Cell Cultures and Vaccinia Virus Stocks. Current protocols in molecular biology. 2017 Jan 05;117:16.16.1-16.16.18