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    With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination-mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology-independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis. © 2017 Wiley Periodicals, Inc.


    Zhaoying Shi, Dandan Tian, Huhu Xin, Jingru Lian, Xiaogang Guo, Yonglong Chen. Targeted integration of genes in Xenopus tropicalis. Genesis (New York, N.Y. : 2000). 2017 Jan;55(1-2)

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    PMID: 28095621

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