An N-end rule pathway that recognizes proline and destroys gluconeogenic enzymes.

Shun-Jia Chen, Xia Wu, Brandon Wadas, Jang-Hyun Oh, Alexander Varshavsky

Science (New York, N.Y.) 2017 Jan 27

filter terms:

Cells synthesize glucose if deprived of it, and destroy gluconeogenic enzymes upon return to glucose-replete conditions. We found that the Gid4 subunit of the ubiquitin ligase GID in the yeast Saccharomyces cerevisiae targeted the gluconeogenic enzymes Fbp1, Icl1, and Mdh2 for degradation. Gid4 recognized the N-terminal proline (Pro) residue and the ~5-residue-long adjacent sequence motifs. Pck1, the fourth gluconeogenic enzyme, contains Pro at position 2; Gid4 directly or indirectly recognized Pro at position 2 of Pck1, contributing to its targeting. These and related results identified Gid4 as the recognition component of the GID-based proteolytic system termed the Pro/N-end rule pathway. Substrates of this pathway include gluconeogenic enzymes that bear either the N-terminal Pro residue or a Pro at position 2, together with adjacent sequence motifs. Copyright © 2017, American Association for the Advancement of Science.

Citation

Shun-Jia Chen, Xia Wu, Brandon Wadas, Jang-Hyun Oh, Alexander Varshavsky. An N-end rule pathway that recognizes proline and destroys gluconeogenic enzymes. Science (New York, N.Y.). 2017 Jan 27;355(6323)