Production of alkenes and novel secondary products by P450 OleTJE using novel H2 O2 -generating fusion protein systems.

Sarah Matthews, Kang Lan Tee, Nicholas J Rattray, Kirsty J McLean, David Leys, David A Parker, Richard T Blankley, Andrew W Munro

FEBS letters 2017 Mar

filter terms:

Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase (AldO) is linked to OleTJE . AldO oxidizes polyols (including glycerol), generating H2 O2 as a coproduct and facilitating its use for efficient OleTJE -dependent fatty acid decarboxylation. AldO activity is regulatable by polyol substrate titration, enabling control over H2 O2 supply to minimize oxidative inactivation of OleTJE and prolong activity for increased alkene production. We also use these fusion systems to generate novel products from secondary turnover of 2-OH and 3-OH myristic acid primary products, expanding the catalytic repertoire of OleTJE . © 2017 Federation of European Biochemical Societies.

Citation

Sarah Matthews, Kang Lan Tee, Nicholas J Rattray, Kirsty J McLean, David Leys, David A Parker, Richard T Blankley, Andrew W Munro. Production of alkenes and novel secondary products by P450 OleTJE using novel H2 O2 -generating fusion protein systems. FEBS letters. 2017 Mar;591(5):737-750