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Prion Transfection of Yeast.
Herman K Edskes, Dmitry Kryndushkin, Frank Shewmaker, Reed B Wickner
Cold Spring Harbor protocols 2017 Feb 01Sizes of these terms reflect their relevance to your search.
Transfection of yeast with amyloid filaments, made from recombinant protein or prepared from extracts of cells infected with a prion, has become an important method in characterizing yeast prions. Here, we describe a method for transmission of [URE3] with Ure2p amyloid that is based on a previously published protocol for transfection with Sup35p filaments to make cells [PSI+]. This method may be used for other prions by changing just the amyloid source, host strain, and plating medium.
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Herman K Edskes, Dmitry Kryndushkin, Frank Shewmaker, Reed B Wickner. Prion Transfection of Yeast. Cold Spring Harbor protocols. 2017 Feb 01;2017(2):pdb.prot089037
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PMID: 28148849
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