Correlation Engine 2.0
Clear Search sequence regions

  • acyl (1)
  • cell (7)
  • cell fusion (3)
  • ceramides (3)
  • cytoplasm (1)
  • DC- STAMP (3)
  • gene (3)
  • glycerophospholipids (2)
  • GTP (2)
  • humans (1)
  • IIA (2)
  • lipids (2)
  • mice (1)
  • Myh9 (8)
  • myosin (4)
  • osteoclast (7)
  • osteogenesis (1)
  • phosphoglycerol dihydroceramide (9)
  • porphyromonas gingivalis (8)
  • Rac1 (3)
  • rac1 protein (1)
  • rank ligand (2)
  • RANKL (3)
  • signal (2)
  • TLR2 (1)
  • Tnfsf11 (1)
  • vitro (2)
  • Sizes of these terms reflect their relevance to your search.

    Among several virulence factors produced by the periodontal pathogen Porphyromonas gingivalis (Pg), a recently identified novel class of dihydroceramide lipids that contains a long acyl-chain has the potential to play a pathogenic role in periodontitis because of its higher level of tissue penetration compared to other lipid classes produced by Pg. However, the possible impact of Pg ceramides on osteoclastogenesis is largely unknown. In the present study, we report that the phosphoglycerol dihydroceramide (PGDHC) isolated from Pg enhanced osteoclastogenesis in vitro and in vivo. Using RAW264.7 cells, in vitro assays indicated that PGDHC can promote RANKL-induced osteoclastogenesis by generating remarkably larger TRAP+ multinuclear osteoclasts compared to Pg LPS in a TLR2/4-independent manner. According to fluorescent confocal microscopy, co-localization of non-muscle myosin II-A (Myh9) and PGDHC was observed in the cytoplasm of osteoclasts, indicating the membrane-permeability of PGDHC. Loss- and gain-of-function assays using RNAi-based Myh9 gene silencing, as well as overexpression of the Myh9 gene, in RAW264.7 cells showed that interaction of PGDHC with Myh9 enhances RANKL-induced osteoclastogenesis. It was also demonstrated that PGDHC can upregulate the expression of dendritic cell-specific transmembrane protein (DC-STAMP), an important osteoclast fusogen, through signaling that involves Rac1, suggesting that interaction of PGDHC with Myh9 can elicit the cell signal that promotes osteoclast cell fusion. Taken together, our data indicated that PGDHC is a Pg-derived, cell-permeable ceramide that possesses a unique property of promoting osteoclastogenesis via interaction with Myh9 which, in turn, activates a Rac1/DC-STAMP pathway for upregulation of osteoclast cell fusion. Copyright © 2017. Published by Elsevier B.V.


    Hiroyuki Kanzaki, Alexandru Movila, Rayyan Kayal, Marcelo H Napimoga, Kenji Egashira, Floyd Dewhirst, Hajime Sasaki, Mohammed Howait, Ayman Al-Dharrab, Abdulghani Mira, Xiaozhe Han, Martin A Taubman, Frank C Nichols, Toshihisa Kawai. Phosphoglycerol dihydroceramide, a distinctive ceramide produced by Porphyromonas gingivalis, promotes RANKL-induced osteoclastogenesis by acting on non-muscle myosin II-A (Myh9), an osteoclast cell fusion regulatory factor. Biochimica et biophysica acta. 2017 May;1862(5):452-462

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 28153611

    View Full Text