Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin.

Sergii Kropyvko, Tetyana Gryaznova, Dmytro Morderer, Alla Rynditch

Biochemical and biophysical research communications 2017 Mar 18

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Actin cytoskeleton rearrangements are required for normal cell functioning, and their deregulation leads to various pathologies. Members of two mammalian protein families - ITSNs (ITSN1 and ITSN2) and verprolins (WIP, CR16 and WIRE) are involved in Cdc42/N-WASP/Arp2/3 signaling pathway-mediated remodeling of the actin cytoskeleton. Recently we demonstrated that ITSNs interact with the actin-regulating protein WIP. Here, we show that other member of verprolin family, CR16, also forms complexes with ITSN1 and ITSN2 in human cell lines. The actin-binding protein CR16 modulates ITSN/β-actin association. Moreover, overexpressed CR16 promoted co-localization of ITSN1 with F-actin in MCF-7 breast cancer cells. Our data demonstrated that CR16 mRNA is expressed in glioblastoma and breast tumors. These findings provide the basis for further functional investigations of the ITSN/CR16 complex that may play an important role in actin remodeling and cellular invasion. Copyright © 2017 Elsevier Inc. All rights reserved.

Citation

Sergii Kropyvko, Tetyana Gryaznova, Dmytro Morderer, Alla Rynditch. Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin. Biochemical and biophysical research communications. 2017 Mar 18;484(4):813-819