Multi-scale characterization of thermoresponsive dendritic elastin-like peptides.

Mingjun Zhou, Yulia Shmidov, John B Matson, Ronit Bitton

Colloids and surfaces. B, Biointerfaces 2017 May 01

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Elastin like peptides (ELPs)-polypeptides based on the protein elastin-are used widely as thermoresponsive components in biomaterials due to the presence of a sharp soluble-to-insoluble phase change at a characteristic transition temperature (Tt). While linear ELPs have been thoroughly studied, few investigations into branched ELPs have been carried out. Using lysine amino acids as branching and terminal units with 1-3 pentameric repeats between each branch, ELP dendrimers were prepared by solid-phase peptide synthesis with molecular weights as high as 14kDa. A conformation change from random coil to β-turn upon heating through the Tt, typical of ELPs, was observed by circular dichroism spectroscopy for all peptides. The high molecular weights of these peptides enabled the use of characterization techniques typically reserved for polymers. Variable-temperature small-angle X-ray scattering measurements in dilute solution revealed an increase in size and fractal dimension upon heating, even well below the Tt. These results were corroborated by cryogenic transmission electron microscopy, which confirmed the presence of aggregates below the Tt, and micro differential scanning calorimetry, which showed a broad endothermic peak below the Tt. These results collectively indicate the presence of a pre-coacervation step in the phase transition of ELP dendrimers. Copyright © 2017 Elsevier B.V. All rights reserved.

Citation

Mingjun Zhou, Yulia Shmidov, John B Matson, Ronit Bitton. Multi-scale characterization of thermoresponsive dendritic elastin-like peptides. Colloids and surfaces. B, Biointerfaces. 2017 May 01;153:141-151