Aggregation of trypsin and trypsin inhibitor by Al cation.

P Chanphai, L Kreplak, H A Tajmir-Riahi

Journal of photochemistry and photobiology. B, Biology 2017 Apr

filter terms:

Al cation may trigger protein structural changes such as aggregation and fibrillation, causing neurodegenerative diseases. We report the effect of Al cation on the solution structures of trypsin (try) and trypsin inhibitor (tryi), using thermodynamic analysis, UV-Visible, Fourier transform infrared (FTIR) spectroscopic methods and atomic force microscopy (AFM). Thermodynamic parameters showed Al-protein bindings occur via H-bonding and van der Waals contacts for trypsin and trypsin inhibitor. AFM showed that Al cations are able to force trypsin into larger or more robust aggregates than trypsin inhibitor, with trypsin 5±1 SE (n=52) proteins per aggregate and for trypsin inhibitor 8.3±0.7 SE (n=118). Thioflavin T test showed no major protein fibrillation in the presence of Al cation. Al complexation induced more alterations of trypsin inhibitor conformation than trypsin. Copyright © 2017 Elsevier B.V. All rights reserved.

Citation

P Chanphai, L Kreplak, H A Tajmir-Riahi. Aggregation of trypsin and trypsin inhibitor by Al cation. Journal of photochemistry and photobiology. B, Biology. 2017 Apr;169:7-12