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Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain. Copyright © 2017, American Association for the Advancement of Science.


Yue Shen, Yun Wang, Tai Chen, Feng Gao, Jianhui Gong, Dariusz Abramczyk, Roy Walker, Hongcui Zhao, Shihong Chen, Wei Liu, Yisha Luo, Carolin A Müller, Adrien Paul-Dubois-Taine, Bonnie Alver, Giovanni Stracquadanio, Leslie A Mitchell, Zhouqing Luo, Yanqun Fan, Baojin Zhou, Bo Wen, Fengji Tan, Yujia Wang, Jin Zi, Zexiong Xie, Bingzhi Li, Kun Yang, Sarah M Richardson, Hui Jiang, Christopher E French, Conrad A Nieduszynski, Romain Koszul, Adele L Marston, Yingjin Yuan, Jian Wang, Joel S Bader, Junbiao Dai, Jef D Boeke, Xun Xu, Yizhi Cai, Huanming Yang. Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome. Science (New York, N.Y.). 2017 Mar 10;355(6329)

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PMID: 28280153

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