Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond.

Science (New York, N.Y.) 2017 Mar 10

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We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to synonymous coding changes within PRE4 (YFR050C), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of 16 synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteomewide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains. Another "bug" was discovered through proteomic analysis, associated with alteration of the HIS2 transcription start due to transfer RNA deletion and loxPsym site insertion. Despite extensive genetic alterations across 6% of the genome, no major global changes were detected in the poly-synthetic strain "omics" analyses. This work sets the stage for completion of a designer, synthetic eukaryotic genome. Copyright © 2017, American Association for the Advancement of Science.

Citation

Leslie A Mitchell, Ann Wang, Giovanni Stracquadanio, Zheng Kuang, Xuya Wang, Kun Yang, Sarah Richardson, J Andrew Martin, Yu Zhao, Roy Walker, Yisha Luo, Hongjiu Dai, Kang Dong, Zuojian Tang, Yanling Yang, Yizhi Cai, Adriana Heguy, Beatrix Ueberheide, David Fenyö, Junbiao Dai, Joel S Bader, Jef D Boeke. Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond. Science (New York, N.Y.). 2017 Mar 10;355(6329)