Generation of Antifungal Stilbenes Using the Enzymatic Secretome of Botrytis cinerea.

The protein secretome of Botrytis cinerea was used to perform the biotransformation of resveratrol, pterostilbene, and a mixture of both. Metabolite profiling by UHPLC-HRMS revealed the presence of compounds with unusual molecular formula, suggesting the existence of new products. To isolate these products, the reactions were scaled-up, and 21 analogues were isolated and fully characterized by NMR and HRESIMS analyses. The reaction with pterostilbene afforded five new compounds, while the reaction with a mixture of pterostilbene and resveratrol afforded seven unusual stilbene dimers. The antifungal properties of these compounds were evaluated using in vitro bioassays against Plasmopara viticola. The cytological effects of the isolated antifungal compounds on the ultrastructure of P. viticola were also evaluated.

Citation

Katia Gindro, Sylvain Schnee, Davide Righi, Laurence Marcourt, Samad Nejad Ebrahimi, Josep Massana Codina, Francine Voinesco, Emilie Michellod, Jean-Luc Wolfender, Emerson Ferreira Queiroz. Generation of Antifungal Stilbenes Using the Enzymatic Secretome of Botrytis cinerea. Journal of natural products. 2017 Apr 28;80(4):887-898

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PMID: 28332842

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